Eugene W. Leake graduated from New York Law School in 1898. He was admitted to the New Jersey bar in 1898 and commenced practice in Jersey City. In 1908, he was admitted to the New York bar and practiced in New York City;
Leake was elected as a Democrat to the Sixtieth Congress, serving in office from March 4, 1907-March 3, 1909, but was not a candidate for renomination in 1908.
After leaving Congress, he was general counsel for the Adams Express Co. from 1927-1932. In 1931, he was elected chairman of the board of directors of the American Railway Express Co., and was a director of Loew's, Inc.